AntiNuclear Antibody (ANA) Test Kit

10x6-Well Slides
 

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• A sensitive and qualitative method to detect antinuclear antibodies in human serum. 
• Utilizes HEp-2 cells containing Mitotic Figures, which are grown and directly stabilized on microscope slides.
• The antinuclear antibodies can aid in the diagnosis of systemic rheumatic diseases such as:
• Systemic Lupus Erythematosus
• Scleroderma
• Sjøgren’s Syndrome
• Mixed connective tissue disease
• Progressive Systemic Sclerosis with CREST Syndrome

The Antibodies Incorporated ANA test is a qualitative method for the detection of antinuclear antibody in human serum.  The method is based upon detection of the reaction between antinuclear antibody and nuclear antigen by means of fluorescent antibody.  The nuclear antigen is provided by human HEp-2 cells which are grown directly on microscope slides.  In a positive reaction, the antinuclear antibody from the serum sample forms an immune complex with the antigen of the cell nucleus.  After washing to remove any unattached human gamma globulin, fluorescent anti-human gamma globulin is added, thus forming a three part complex.  The slide is again washed to remove unattached fluorescent anti-human gamma globulin and then examined with a fluorescent microscope.  If the nuclei fluoresce more brightly than the negative control well with a discernible pattern, the reaction is considered positive.  A negative reaction will not show fluorescent nuclei as there will be no antinuclear antibody attachment to the cells.