Fetal Cell Count Kit

25 tests

*Please use promo #CL08-1 when ordering to receive discount.
 

Price:

$389.00

 

The Fetal Cell Count™ kit is intended for the discrimination and quantitative detection of human fetal red blood cells in maternal blood. The Fetal Cell Count™ kit is based on a sensitive and accurate flow cytometric method, which offers a dual fluorescent detection of two intracellular antigens, Hemoglobin F (HbF) and Carbonic Anhydrase (CA). Both HbF and CA are detected in red blood cells obtained from EDTA anti-coagulated or Heparin-treated human peripheral whole blood. The complete dual-color staining and analysis of up to 5 samples can be concluded within 1.5 hour from blood collection.

Principle of the test

The Fetal Cell Count™ methodology is based on a combination of two antibodies. One is directed against fetal hemoglobin (HbF), which is present in fetal RBCs and in a small percentage of adult RBCs (called F-cells). The second antibody is directed against Carbonic Anhydrase (CA), an enzyme only present in adult RBCs and very late stage fetal cells. The dual-color flow cytometric method allows simultaneous detection of these two intracellular antigens, while the use of formaldehyde as fixative and sodium dodecyl sulfate (SDS) for permeabilization of fixed RBCs results in low background staining, negligible HbF leakage, and minimal cell clumping.

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